Produced from sera of rabbits pre-immunized with highly pure recombinant Human 4-1BBL. Anti-Human 4-1BBL specific antibody was purified by affinity chromatography employing immobilized Human 4-1BBL matrix.
E.coli derived Recombinant Human 4-1BB Ligand (PeproTech catalog# 310-11)
To detect h4-1BBL by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human 4-1BBL (500-P169Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant h4-1BBL.
To detect h4-1BBL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant h4-1BBL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.