Produced in BALB/c mice immunized with highly pure Recombinant Human CRP. Anti-Human CRP-specific antibody was purified from cell culture supernatant by Protein A affinity chromatography.
E.coli-derived, 23.2 kDa Recombinant Human CRP (PeproTech catalog# 350-13)*
*Catalog# 350-13 has been discontinued.
In a sandwich ELISA (assuming 100μl/well), a concentration of 8.0μg/ml of this antibody will detect at least 16ng/ml of Recombinant Human CRP when used in conjunction with compatible secondary reagents.
To detect Human CRP by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human CRP is 0.20-0.40 ng/lane, under non-reducing conditions and 2.0-4.0 ng/lane, under reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.