Produced in BALB/c mice immunized with highly pure Recombinant Human MIP-1α (CCL3). Anti-Human MIP-1α (CCL3)-specific antibody was purified from cell culture by Protein G affinity chromatography.
E.coli derived Recombinant Human MIP-1α (CCL3) (PeproTech catalog# 300-08)
In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 2000pg/ml of Recombinant Human MIP-1α when used with PeproTech’s Biotinylated Antigen Affinity Purified anti-Human MIP-1α (CCL3) (500-P38GBT) as the detection antibody at a concentration of approximately 1.0-2.0 μg/ml.
To detect Human MIP-1α by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MIP-1α is 1.0-2.0 ng/lane, under non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.