Produced in BALB/c mice immunized with highly pure Recombinant Human TNF-α. Anti-Human TNF- α-specific antibody was purified from cell culture by Protein G affinity chromatography.
E.coli derived Recombinant Human TNF-α (PeproTech catalog# 300-01A)
In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 1000 pg/ml of Recombinant Human TNF-α when used with PeproTech’s Biotinylated Antigen Affinity Purified Anti-Human TNF-α (500-P31ABT) as the detection antibody at a concentration of at least 0.5-1.0 μg/ml.
To detect Human TNF-α by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human TNF-α is 0.5-1.0 ng/lane, under reducing conditions and 0.25-0.5 ng/lane, under non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.