Produced in BALB/c mice immunized with highly pure Recombinant Human VEGF165. Anti-Human VEGF165-specific antibody was purified from cell culture by Protein A affinity chromatography.
E.coli derived Recombinant Human VEGF165 (PeproTech catalog# 100-20)
In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 100pg/ml of Recombinant Human VEGF when used with PeproTech’s Biotinylated Antigen Affinity Purified anti-Human VEGF (500-P10BT) as the detection antibody at a concentration of approximately 0.25- 1.0 μg/ml.
To detect Human VEGF by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant HumanVEGF is 0.25-0.50 ng/lane, under non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.