Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-1α. Anti-Murine IL-1α specific antibody was purified by affinity chromatography employing immobilized mIL-1α matrix.
E.coli derived Recombinant Murine IL-1α (PeproTech catalog# 211-11A)
To detect mIL-1α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Murine IL-1α (500-P51ABt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-1α.
To detect mIL-1α by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1α is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.