Recombinant Human Adiponectin 0 评论Submit a Review 产品详情 产品编号: 450-24 产品描述: Adiponectin is an adipose-derived secreted protein containing 226 amino acid residues. It is relatively abundant in humans and rodents, accounting for about 0.01% of total plasma protein. The circulating levels of adiponectin are decreased under conditions of obesity, insulin resistance, and type II diabetes. Disruption of adiponectin in mice causes insulin resistance and neointimal formation. Conversely, administration of recombinant adiponectin suppresses hepatic glucose production, and reverses insulin resistance associated with both lipoatrophy and obesity. The protective role of adiponectin is attributed to its anti-inflammatory properties (e.g. ability to suppress expression of TNF-α and class A scavenger receptor in macrophages). Recombinant Human Adiponectin is a multimeric glycoprotein containing amino acids Glu-19 to Asn-244 of the adiponectin precursor protein fused to an N-terminal histidine tag. Monomeric glycosylated adiponectin migrates at an apparent molecular weight of approximately 35.0 kDa by SDS PAGE analysis under reducing conditions. The calculated molecular weight of Recombinant Human Adiponectin is 25.9 kDa. Source: Hi-5 Insect cells Synonyms: Acrp-30, GBP-28, APM-1, Gelatin-binding protein, AdipoQ AA Sequence: RGHHHHHHHH ETTTQGPGVL LPLPKGACTG WMAGIPGHPG HNGAPGRDGR DGTPGEKGEK GDPGLIGPKG DIGETGVPGA EGPRGFPGIQ GRKGEPGEGA YVYRSAFSVG LETYVTIPNM PIRFTKIFYN QQNHYDGSTG KFHCNIPGLY YFAYHITVYM KDVKVSLFKK DKAMLFTYDQ YQENNVDQAS GSVLLHLEVG DQVWLQVYGE GERNGLYADN DNDSTFTGFL LYHDTN Purity: ≥ 97% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by a cytotoxic assay using M1 cells. The ED50 for this effect is 3.0-6.0 μg/ml. Calculated Molecular Weight: 25.9 kDa Accession Number: Q15848 Gene ID: 9370 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) Note: 1mg will be provided as 2x500μg. crossreactivity: Human, Mouse, Rat References PubMed SDS CoA 搜索 Product Line Country Of Origin: USA Not for human use. Research Interest 糖尿病/体重调节 免疫系统 炎症 神经生物学 product.subtitle.recentcitations 第一作者 Spinnler, R 标题 The adipocytokine Nampt and its product NMN have no effect on beta-cell survival but potentiate glucose stimulated insulin secretion. 文献引用 PLoS ONE; 8(1) pge54106 PubMed Id 23342086 第一作者 Wijesekara, N 标题 Adiponectin-induced ERK and Akt phosphorylation protects against pancreatic beta cell apoptosis and increases insulin gene expression and secretion. 文献引用 The Journal of Biological Chemistry; 285(44) pg33623-31 PubMed Id 20709750 第一作者 Kirouac, D C 标题 Dynamic interaction networks in a hierarchically organized tissue. 文献引用 Molecular Systems Biology; 6 pg417 PubMed Id 20924352