Recombinant Human GDNF 3 评论Submit a Review 产品详情 产品编号: 450-10 产品描述: GDNF is a disulfide-linked, homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors. GDNF specifically promotes dopamine uptake and survival, and morphological differentiation of midbrain neurons. Using a Parkinson’s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. The functional human GDNF ligand is a disulfide-linked homodimer consisting of two 15 kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, including Cys-101, which is used for inter-chain disulfide bridging, and others that are involved in the intramolecular ring formation known as the cysteine-knot configuration. The calculated molecular weight of Recombinant Human GDNF is 30.4 kDa. Source: E.coli Synonyms: Glial-Derived Neurotrophic Factor, ATF-1 AA Sequence (monomer): MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by a cell proliferation assay using SH-SY5Y cells. The expected ED50 for this effect is 1.0-10.0 ng/ml. This product has been tested against the WHO standard. International Unit information can be found here. Calculated Molecular Weight: 30.4 kDa Accession Number: P39905 Gene ID: 2668 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) crossreactivity: Bird, Frog, Human, Human + Hamster, Human + Mouse, Human + Rat, Human + Virus, Monkey, Mouse, Rat References PubMed SDS CoA 搜索 Product Line Country Of Origin: USA Not for human use. Research Interest Organoids 血管生成/心血管 细胞培养 免疫系统 神经生物学 干细胞&分化 TGF-β超家族 product.subtitle.recentcitations 第一作者 Pantazis, C B 标题 A reference human induced pluripotent stem cell line for large-scale collaborative studies. 文献引用 Cell Stem Cell; 29(12) pg1685-1702.e22 PubMed Id 36459969 第一作者 Kaluscha, S 标题 Evidence that direct inhibition of transcription factor binding is the prevailing mode of gene and repeat repression by DNA methylation. 文献引用 Nature Genetics; 54(12) pg1895-1906 PubMed Id 36471082 第一作者 Mesci, P 标题 SARS-CoV-2 infects human brain organoids causing cell death and loss of synapses that can be rescued by treatment with Sofosbuvir. 文献引用 PLoS Biology; 20(11) pge3001845 PubMed Id 36327326 product.subtitle.productreviews 现有评论 High quality product Used for human stem cells. Awesome product! 来自: Danielle | 日期: 2020/8/26 × High quality product Used for human stem cells. Awesome product! 来自: Danielle | 日期: 2020/8/26 Close Cortical Neuron Differentiation Very consistent for culturing human stem cells 来自: Danielle | 日期: 2021/8/24 × Cortical Neuron Differentiation Very consistent for culturing human stem cells 来自: Danielle | 日期: 2021/8/24 Close iPSC-derived neurons Used successfully as part of a battery of growth factors and small molecules to differentiate iPSCs into neurons. 来自: Mandi | 日期: 2021/8/25 × iPSC-derived neurons Used successfully as part of a battery of growth factors and small molecules to differentiate iPSCs into neurons. 来自: Mandi | 日期: 2021/8/25 Close