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无动物成分细胞因子

Animal-Free Recombinant Human IGF-I

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Product Details

Catalogue Number: AF-100-11
Description:
Animal-Free Recombinant Human IGF-I

The IGFs are mitogenic, polypeptide growth factors that stimulate the proliferation and survival of various cell types, including muscle, bone, and cartilage tissue in vitro.  IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times.  The IGFs belong to the Insulin gene family, which also contains insulin and relaxin.  The IGFs are similar to insulin by structure and function, but have a much higher growth-promoting activity than insulin.  IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone.  Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor.  Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions.  Recombinant Human IGF-I and IGF-II are globular proteins containing 70 and 67 amino acids, respectively, and 3 intra-molecular disulfide bonds. The calculated molecular weight of Recombinant Human IGF-I is 7.6 kDa.     

Manufactured using all Animal-Free reagents.

Source: E.coli

Synonyms: Insulin-like Growth Factor-I, Somatamedin C, IGF-IA

AA Sequence: GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED50 was determined by a cell proliferation assay using FDC-P1 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Animal-Free Recombinant Human IGF-I WHO Comparison Graph Animal-Free Recombinant Human IGF-I WHO Comparison Graph

Calculated Molecular Weight: 7.6 kDa

Accession Number: P05019

Gene ID: 3479

Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug)

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Sissaoui, S
Title
Hyperglycaemia up-regulates placental growth factor (PlGF) expression and secretion in endothelial cells via suppression of PI3 kinase-Akt signalling and activation of FOXO1
Citation
Scientific Reports; 11(1) pg16344
PubMed Id
First Author
James, O G
Title
iPSC-derived myelinoids to study myelin biology of humans.
Citation
Developmental Cell; 56(9) pg1346-1358.e6
PubMed Id
First Author
García-León, J A
Title
Generation of oligodendrocytes and establishment of an all-human myelinating platform from human pluripotent stem cells.
Citation
Nature Protocols; 15(11) pg3716-3744
PubMed Id