Recombinant Human SPARC/Osteonectin 0 评论Submit a Review 产品详情 产品编号: 120-36 产品描述: SPARC/Osteonectin is a secreted, evolutionarily-conserved, collagen-binding glycoprotein that is involved in a variety of cellular activities. It is highly expressed in tissues undergoing morphogenesis, remodeling and wound repair. SPARC/Osteonectin and its related peptides bind to numerous proteins of the extracellular matrix (ECM), affect ECM protein expression, influence cellular adhesion and migration, and modulate growth factor-induced cell proliferation and angiogenesis. SPARC/Osteonectin consists of three domains: an N-terminal acidic region that binds calcium ions with low affinity, a module containing two EF-hand motifs that bind calcium with high affinity, and a cysteine-rich follistatin-like domain. Recombinant Human SPARC/Osteonectin is a glycoprotein containing 286 amino acids that migrates at an apparent MW of 43.7 kDa by SDS-PAGE analysis due to the effect of glycosylation. The calculated molecular weight of Recombinant Human SPARC/Osteonectin is 32.7 kDa. Source: CHO cells Synonyms: Secreted protein acidic and rich in cysteine, BM-40, ON AA Sequence: APQQEALPDE TEVVEETVAE VTEVSVGANP VQVEVGEFDD GAEETEEEVV AENPCQNHHC KHGKVCELDE NNTPMCVCQD PTSCPAPIGE FEKVCSNDNK TFDSSCHFFA TKCTLEGTKK GHKLHLDYIG PCKYIPPCLD SELTEFPLRM RDWLKNVLVT LYERDEDNNL LTEKQKLRVK KIHENEKRLE AGDHPVELLA RDFEKNYNMY IFPVHWQFGQ LDQHPIDGYL SHTELAPLRA PLIPMEHCTT RFFETCDLDN DKYIALDEWA GCFGIKQKDI DKDLVI Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by its ability to inhibit alkaline phosphatase activity in differentiating MC3T3 cells, with an IC50 of 0.5-0.7 μg/ml. Calculated Molecular Weight: 32.7 kDa Accession Number: P09486 Gene ID: 6678 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) crossreactivity: Human, Mouse, Rat References PubMed SDS CoA 搜索 Product Line Country Of Origin: USA Not for human use. Research Interest 血管生成/心血管 骨/骨骼系统/软骨 癌症 免疫系统 创伤修复 product.subtitle.recentcitations 第一作者 Yao, L 标题 Bidirectional epithelial-mesenchymal crosstalk provides self-sustaining profibrotic signals in pulmonary fibrosis. 文献引用 The Journal of Biological Chemistry; 297(3) pg101096 PubMed Id 34418430 第一作者 Zhu, J 标题 SPARC promotes self-renewal of limbal epithelial stem cells and ocular surface restoration through JNK and p38-MAPK signaling pathways. 文献引用 Stem Cells; 38(1) pg134-145 PubMed Id 31644832 第一作者 John, B 标题 Regulation of the bi-directional cross-talk between ovarian cancer cells and adipocytes by SPARC. 文献引用 Oncogene; 38(22) pg4366-4383 PubMed Id 30765860