Animal-Free Recombinant Human TPO 0 评论Submit a Review 产品详情 产品编号: AF-300-18 产品描述: TPO is a lineage-specific growth factor produced in the liver, kidney and skeletal muscle. It stimulates the proliferation and maturation of megakaryocytes, and promotes increased circulating levels of platelets in vivo. TPO signals through the c-mpl receptor, and acts as an important regulator of circulating platelets. Human and murine TPO exhibit cross-species reactivity. The human TPO gene encodes for a 353 amino acid glycoprotein, which contains a 21 amino acid signal sequence, a 15 amino acid erythropoietin-like domain, and a highly glycosylated 179 amino acid C-terminal domain. Recombinant Human TPO is a fully biologically active 174 amino acid polypeptide (18.6 kDa), which contains the erythropoietin-like domain of the full length TPO protein. Manufactured using all Animal-Free reagents. Source: E.coli Synonyms: Thrombopoietin, Megakaryocyte colony-stimulating factor, c-MPL Ligand, MGDF AA Sequence: SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of human MO7e cells was found to be ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg. Calculated Molecular Weight: 18.6 kDa Accession Number: P40225 Gene ID: 7066 Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug) crossreactivity: Human, Human + Hamster, Monkey, Mouse, Rat References PubMed SDS CoA 搜索 Product Line Country Of Origin: USA Not for human use. Research Interest 细胞培养 免疫系统 干细胞&分化 创伤修复 product.subtitle.recentcitations 第一作者 Eernstman, J 标题 Novel variants in Krueppel like factor 1 that cause persistence of fetal hemoglobin in In(Lu) individuals 文献引用 Scientific Reports; 11(1) pg18557 PubMed Id 34535703 第一作者 McGrath, E 标题 Targeting specificity of APOBEC-based cytosine base editor in human iPSCs determined by whole genome sequencing. 文献引用 Nature Communications; 10(1) pg5353 PubMed Id 31767844 第一作者 Minuesa, G 标题 Small-molecule targeting of MUSASHI RNA-binding activity in acute myeloid leukemia. 文献引用 Nature Communications; 10(1) pg2691 PubMed Id 31217428