Animal-Free Recombinant Murine Noggin 0 评论Submit a Review 产品详情 产品编号: AF-250-38 产品描述: Noggin belongs to a group of diffusible proteins that bind to ligands of the TGF-β family, and regulate their activity by inhibiting their access to signaling receptors. Noggin was originally identified as a BMP-4 antagonist whose action was critical for proper formation of the head and other dorsal structures. Consequently, noggin has been shown to modulate the activities of other BMPs including BMP-2,-7,-13, and -14. Targeted deletion of noggin in mice results in prenatal death, and a recessive phenotype displaying a severely malformed skeletal system. Conversely, transgenic mice over-expressing noggin in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis. Recombinant Murine Noggin is a 46.4 kDa disulfide-linked homodimer consisting of two 206 amino acid polypeptide chains. Manufactured using all Animal-Free reagents. Source: E.coli Synonyms: None AA Sequence (monomer): MQHYLHIRPA PSDNLPLVDL IEHPDPIFDP KEKDLNETLL RSLLGGHYDP GFMATSPPED RPGGGGGPAG GAEDLAELDQ LLRQRPSGAM PSEIKGLEFS EGLAQGKKQR LSKKLRRKLQ MWLWSQTFCP VLYAWNDLGS RFWPRYVKVG SCFSKRSCSV PEGMVCKPSK SVHLTVLRWR CQRRGGQRCG WIPIQYPIIS ECKCSC Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC5 chondrogenic cells. The expected ED50 for this effect is 1.0-2.0 ng/ml of Noggin. Calculated Molecular Weight: 46.4 kDa Accession Number: P97466 Gene ID: 18121 Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug) Note: 1mg will be provided as 2x500μg. crossreactivity: Human, Mouse References PubMed SDS CoA 搜索 Product Line Country Of Origin: USA Not for human use. Research Interest Organoids 骨/骨骼系统/软骨 免疫系统 神经生物学 干细胞&分化 product.subtitle.recentcitations 第一作者 Lu, J 标题 Characterization of an in vitro 3D intestinal organoid model by using massive RNAseq-based transcriptome profiling 文献引用 Scientific Reports; 11(1) pg16668 PubMed Id 34404908 第一作者 Yamazaki, D 标题 Role of adenomatous polyposis coli in proliferation and differentiation of colon epithelial cells in organoid culture 文献引用 Scientific Reports; 11(1) pg3980 PubMed Id 33597597 第一作者 Osumi, H 标题 Evaluation of the RAS signaling network in response to MEK inhibition using organoids derived from a familial adenomatous polyposis patient 文献引用 Scientific Reports; 10(1) pg17455 PubMed Id 33060766