Animal-Free Recombinant Murine TNF-α 0 评论Submit a Review 产品详情 产品编号: AF-315-01A 产品描述: TNF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells, including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands, and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells, and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation, and diabetes. Human and murine TNF-α demonstrate significant cross-species reactivity. TNF-α exists in two forms; a type II transmembrane protein, and a mature soluble protein. The TNF-α transmembrane protein is proteolitically cleaved to yield a soluble, biologically active, 17 kDa TNF-α, which forms a non-covalently linked homotrimer in solution. Recombinant Murine TNF-α is a soluble 157 amino acid protein (17.3 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein. Manufactured using all Animal-Free reagents. Source: E.coli Synonyms: Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin AA Sequence: MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by the cytolysis of murine L929 cells in the presence of Actinomyocin D. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg. Calculated Molecular Weight: 17.3 kDa Accession Number: P06804 Gene ID: 21926 Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug) crossreactivity: Mouse References PubMed SDS CoA 搜索 Product Line Country Of Origin: USA Not for human use. Research Interest COVID-19 艾滋病/HIV 血管生成/心血管 细胞凋亡 癌症 糖尿病/体重调节 免疫系统 炎症 神经生物学 干细胞&分化 TNF超家族 过敏 移植 product.subtitle.recentcitations 第一作者 Sonner, J 标题 Dietary tryptophan links encephalogenicity of autoreactive T cells with gut microbial ecology. 文献引用 Nature Communications; 10(1) pg4877 PubMed Id 31653831 第一作者 Mandal, P 标题 Caspase-8 Collaborates with Caspase-11 to Drive Tissue Damage and Execution of Endotoxic Shock. 文献引用 Immunity; 49(1) pg42-55.e6 PubMed Id 30021146 第一作者 Pepin, M 标题 Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity. 文献引用 Scientific Reports; 6 pg37953 PubMed Id 27892504