Recombinant Human R-Spondin-3 1 ReviewsSubmit a Review Product Details Catalog Number: 120-44 Description: The R-Spondin (Rspo) proteins belong to the Rspo family of Wnt modulators. Currently, the family consists of four structurally-related, secreted ligands (Rspo 1-4), all containing furin-like and thrombospondin structural domains. The Rspo proteins can interact with the Frizzled/LRP6 receptor complex in a manner that causes the stabilization, and resulting accumulation, of the intracellular signaling protein, β-catenin. This activity effectively activates and increases the subsequent nuclear signaling of β-catenin. R-Spondin can also bind to the previously discovered G-protein coupled receptors, LGR-4 and LGR-5. Rspo/β-catenin signaling can act as an inducer of the transformed phenotype, and can also regulate the proliferation and differentiation of certain stem cell populations. Recombinant Human R-Spondin-3 is a 26.9 kDa protein consisting of 240 amino acid residues. Due to glycosylation, R-Spondin-3 migrates at an apparent molecular weight of approximately 37.0 kDa by SDS PAGE analysis under reducing conditions. Source: CHO cells Synonyms: Roof plate-specific spondin-3, RSPO3, PWTSR, THSD2 AA Sequence: MHPNVSQGCQ GGCATCSDYN GCLSCKPRLF FALERIGMKQ IGVCLSSCPS GYYGTRYPDI NKCTKCKADC DTCFNKNFCT KCKSGFYLHL GKCLDNCPEG LEANNHTMEC VSIVHCEVSE WNPWSPCTKK GKTCGFKRGT ETRVREIIQH PSAKGNLCPP TNETRKCTVQ RKKCQKGERG KKGRERKRKK PNKGESKEAI PDSKSLESSK EIPEQRENKQ QQKKRKVQDK QKSVSVSTVH Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses. Biological Activity: R-Spondin-3 enhances BMP-2-mediated differentiation of MC3T3-E1 cells. The expected ED50 for this effect is 0.8 – 2.0 μg/ml. Calculated Molecular Weight: 26.9 kDa Accession Number: Q9BXY4 Gene ID: 84870 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) Cross Reactivity Cited in References: Human, Mouse References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Angiogenesis/Cardiovascular Lipid Metabolism Stem Cells & Differentiation Recent Citations First Author Lee, H Title R-spondins are BMP receptor antagonists in Xenopus early embryonic development Citation Nature Communications; 11(1) pg5570 PubMed ID 33149137 First Author Sigal, M Title Stromal R-spondin orchestrates gastric epithelial stem cells and gland homeostasis. Citation Nature; 548(7668) pg451-455 PubMed ID 28813421 First Author Yuri, S Title In Vitro Propagation and Branching Morphogenesis from Single Ureteric Bud Cells. Citation Stem Cell Reports; 8(2) pg401-416 PubMed ID 28089670 Product Reviews Existing reviews Organoid Culture Realiable and fast delivery as always. The component is used in organoid culture. From: Iris | Date: 8/23/2021 × Organoid Culture Realiable and fast delivery as always. The component is used in organoid culture. From: Iris | Date: 8/23/2021 Close