Recombinant Human SCF 2 评论Submit a Review 产品详情 产品编号： 300-07 产品描述： SCF is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages. Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells. The human SCF gene encodes for a 273 amino acid transmembrane protein, which contains a 25 amino acid N-terminal signal sequence, a 189 amino acid extracellular domain, a 23 amino acid transmembrane domain, and a 36 amino acid cytoplasmic domain. The secreted soluble form of SCF is generated by proteolytic processing of the membrane anchored precursor. Recombinant Human SCF is an 18.4 kDa polypeptide containing 165 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF. Source: E.coli Synonyms: Stem Cell Factor, c-Kit Ligand, Mast Cell Growth Factor (MGF), Steel Factor AA Sequence: MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg. This product has been tested against the WHO standard. International Unit information can be found here. Calculated Molecular Weight: 18.4 kDa Accession Number: P21583 Gene ID: 4254 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) crossreactivity: Bacteria, Human, Human + Hamster, Human + Mouse, Human + Virus, Leech, Monkey, Mouse, Pig, Rabbit, Rat References PubMed SDS CoA 搜索 Product Line Country Of Origin: USA Not for human use. Research Interest 细胞培养 免疫系统 干细胞&分化 product.subtitle.recentcitations 第一作者 Li, Y 标题 Modulation of WNT, Activin/Nodal, and MAPK Signaling Pathways Increases Arterial Hemogenic Endothelium and Hematopoietic Stem/Progenitor Cell Formation During Human iPSC Differentiation. 文献引用 Stem cells (Dayton, Ohio); 41(7) pg685-697 PubMed Id 37220178 第一作者 Chen, W 标题 Reprogramming of human peripheral blood mononuclear cells into induced mesenchymal stromal cells using non-integrating vectors. 文献引用 Communications Biology; 6(1) pg393 PubMed Id 37041280 第一作者 Thomas, G E 标题 The metabolic enzyme hexokinase 2 localizes to the nucleus in AML and normal haematopoietic stem and progenitor cells to maintain stemness. 文献引用 Nature Cell Biology; 24(6) pg872-884 PubMed Id 35668135 product.subtitle.productreviews 现有评论 Most reliable As a huge lab at UCLA, we have been using Peprotech products consistently, this has been a long history of buying hSCF, and the quality has been consistent this whole time. 来自: Marlene | 日期: 2020/7/8 × Most reliable As a huge lab at UCLA, we have been using Peprotech products consistently, this has been a long history of buying hSCF, and the quality has been consistent this whole time. 来自: Marlene | 日期: 2020/7/8 Close Optimal performance for CD34+ cell culture Used for human CD34+ in vitro expansion culture, Peprotech hSCF performs as expected and yields optimal HSPC expansion while retaining phenotypic and functional HSC features. 来自: Gabriele | 日期: 2021/8/3 × Optimal performance for CD34+ cell culture Used for human CD34+ in vitro expansion culture, Peprotech hSCF performs as expected and yields optimal HSPC expansion while retaining phenotypic and functional HSC features. 来自: Gabriele | 日期: 2021/8/3 Close
