Produced in BALB/c mice immunized with highly pure Recombinant Human Adiponectin. Anti-Human Adiponectin-specific antibody was purified from cell culture by Protein A affinity chromatography.
Hi-5 Insect cells derived Recombinant Human Adiponectin (PeproTech catalog# 450-24)
In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 500pg/ml of Recombinant Human Adiponectin when used in conjunction with compatible detection reagents at a concentration of approximately 0.5-1.0 μg/ml.
To detect Human Adiponectin by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Adiponectin is 0.5-1.0 ng/lane, under reducing conditions and 1.0-2.0 ng/lane, under non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.