Antigen Affinity Purified Polyclonal Antibodies

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hNAP-2. Anti-Human NAP-2 specific antibody was purified by affinity chromatography employing immobilized hNAP-2 matrix.

Immunogen: E.coli derived Recombinant Human NAP-2 (CXCL7) (PeproTech catalog# 300-14)

Sandwich ELISA: To detect Human NAP-2 by sandwich ELISA (using 100μl/well) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human NAP-2 (CXCL7) (500-P03BT) as a detection antibody, allows the detection of at least 2000- 4000 pg/ml of Recombinant Human NAP-2.

Western Blot: To detect Human NAP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human NAP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.