To detect Human p16-INK4a-TAT by Western Blot analysis, this antibody can be used at a concentration of 0.1 - 0.2 μg/ml.When used in conjunction with compatible development reagents, the detection limit for recombinant Human p16-INK4a-TAT is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Human p16-INK4a-TAT by sandwich ELISA (using 100 μl/well), a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody,in conjunction with PeproTech’s Polyclonal Anti-Human p16-INK4a-TAT (500-P284) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of Recombinant Human p16-INK4a-TAT.
Produced from sera of rabbits immunized with highly pure recombinant Human p16-INK4a-TAT. Anti-Human p16-INK4a-TAT specific antibody was purified by affinity chromatography and then biotinylated.