Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat EGF (Rat Epidermal Growth Factor). Anti-Rat EGF specific antibody was purified by affinity chromatography employing immobilized Rat EGF matrix.
E.coli derived Recombinant Rat EGF (PeproTech catalog# 400-25)
To detect Rat EGF by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Rat EGF (500-P277Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat EGF.
To detect Rat EGF by Western Blot analysis this antibody can be used at a concentration of 0.5 - 1.0 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat EGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.