Produced from sera of rabbits immunized with highly
pure recombinant Rat
IL-7. Anti-Rat IL-7 specific
antibody was purified by affinity chromatography employing an immobilized Rat
E.coli derived Recombinant Rat IL-7 (PeproTech catalog# 400-07)
detect Rat IL-7 by sandwich
ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of
this antibody is required. This antigen affinity purified antibody, in
conjunction with PeproTech’s Biotinylated Anti-Rat IL-7 (500-P310Bt) as a detection antibody, allows the detection of
at least 0.2 - 0.4 ng/well of recombinant Rat IL-7.
To detect Rat IL-7 by
Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2
μg/ml. When used in conjunction with
compatible secondary reagents the detection limit for recombinant Rat IL-7 is
1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.