Biotinylated Polyclonal Antibodies

Biotinylated Anti-Human IGF-BP3

Product Details

Catalog Number: 500-P230BT

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIGF-BP3. Anti-Human IGF-BP3 specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: E.coli derived Recombinant Human IGF-BP3 (PeproTech catalog# 100-08)

Sandwich ELISA: To detect hIGF-BP3 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human IGF-BP3 (500-P230) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIGF-BP3.

Biotinylated Anti-Human IGF-BP3 Sandwich ELISA

Western Blot: To detect hIGF-BP3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Biotinylated Anti-Human IGF-BP3 Western Blot Reduced Biotinylated Anti-Human IGF-BP3 Western Blot Unreduced


1mg will be provided as 2x500μg.

Research Interest

Country Of Origin: USA

Research Use Only: Not for use in diagnostic or therapeutic procedures.