Produced from sera of rabbits immunized with
highly pure recombinant Rat IL-7. Anti-Rat
IL-7 specific antibody was purified by affinity chromatography and then
E.coli derived Recombinant Rat IL-7 (PeproTech catalog# 400-07)
To detect Rat IL-7 by sandwich ELISA (using 100 ml/well) a concentration of 0.25 – 1.0 μg/ml of this
antibody is required. This biotinylated polyclonal antibody, in conjunction
with PeproTech’s Polyclonal Anti-Rat IL-7 (500-P310) as a capture antibody,
allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat IL-7.
To detect Rat IL-7 by Western Blot analysis this
antibody can be used at a concentration of 0.1 - 0.2 mg/ml. When used in conjunction with compatible
development reagents the detection limit for recombinant Rat IL-7 is 1.5 – 3.0
ng/lane, under either reducing or non-reducing conditions.