Produced from sera of rabbits immunized with highly pure Recombinant Human sCD34. Anti-Human sCD34-specific antibody was purified by affinity chromatography employing an immobilized Human sCD34 matrix.
E.coli-derived, 27.4 kDa Recombinant Human sCD34 (PeproTech Catalog# 310-31)
To detect Human sCD34 by sandwich ELISA (using 100μl/well), a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human sCD34 (500-P327) as a capture antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human sCD34.
To detect Human sCD34 by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human sCD34 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.