Produced from sera of rabbits immunized with highly pure recombinant Human CTACK. Anti-Human CTACK specific antibody was purified by affinity chromatography and then biotinylated.
E.coli derived Recombinant Human CTACK (CCL27) (PeproTech catalog# 300-54)
To detect Human CTACK by sandwich ELISA (using 100 ml/well) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti- Human CTACK (500-P294) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human CTACK.
To detect Human CTACK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. When used in conjunction with compatible development reagents the detection limit for recombinant Human CTACK is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
1mg will be provided as 2x500μg.