Anti-Human IGF-I 0 ReviewsSubmit a Review Product Details Catalog Number: 500-P11 Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IGF-I. Anti-Human IGF-I-specific antibody was purified by affinity chromatography employing immobilized Human IGF-I matrix. Immunogen: E.coli derived Recombinant Human IGF-I (PeproTech catalog# 100-11) Sandwich ELISA: To detect hIGF-I by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human IGF-I (500-P11Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-I. Western Blot: To detect hIGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-I is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Note: Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information. Cross Reactivity Cited in References: Human, Mouse, Rat References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Angiogenesis/Cardiovascular Bone, Skeletal, Cartilage Diabetes/Weight Regulation Inflammation Proliferation Wound Healing Recent Citations First Author Annibalini, G Title The intrinsically disordered E-domains regulate the IGF-1 prohormones stability, subcellular localisation and secretion. Citation Scientific Reports; 8(1) pg8919 PubMed ID 29891966 First Author Jeschke, M G Title The structure and composition of liposomes can affect skin regeneration, morphology and growth factor expression in acute wounds. Citation Gene Therapy; 12(23) pg1718-24 PubMed ID 16034455 First Author Jeschke, M G Title Exogenous liposomal IGF-I cDNA gene transfer leads to endogenous cellular and physiological responses in an acute wound. Citation American journal of physiology. Regulatory, integrative and comparative physiology; 286(5) pgR958-66 PubMed ID 15068969