Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hNAP-2. Anti-Human NAP-2 specific antibody was purified by affinity chromatography and then biotinylated.
To detect Human NAP-2 by sandwich ELISA (using 100μl/well) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human NAP-2 (CXCL7) (500-P03) as a capture antibody, allows the detection of at least 2000 – 4000 pg/ml of Recombinant Human NAP-2.
To detect Human NAP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents the detection limit for Recombinant Human NAP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.