Anti-Human Nanog 1 AvisSubmit a Review Détails du Produit Catalog Number: 500-P236 Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hNanog. Anti-Human Nanog specific antibody was purified by affinity chromatography employing immobilized hNanog matrix. Immunogen: E.coli derived Recombinant Human Nanog (PeproTech catalog# 120-21) Sandwich ELISA: To detect hNanog by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human Nanog (500-P236Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNanog. Western Blot: To detect hNanog by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNanog is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Note: Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information. crossreactivity: Cow, Dog, Human, Mouse, Mouse + Pig, Pig, Salamander References SDS Recherche de CoA Product Line Country Of Origin: USA Not for human use. Research Interest Stem Cells & Differentiation product.subtitle.recentcitations Premier Auteur Xu, W Titre Methanol fixed feeder layers altered the pluripotency and metabolism of bovine pluripotent stem cells. Citation Scientific Reports; 12(1) pg9177 Id PubMed 35654935 Premier Auteur Li, N Titre p57 Suppresses the Pluripotency and Proliferation of Mouse Embryonic Stem Cells by Positively Regulating p53 Activation. Citation Stem Cells International; 2021 pg4968649 Id PubMed 34976070 Premier Auteur De Clerck, L Titre Untargeted histone profiling during naive conversion uncovers conserved modification markers between mouse and human. Citation Scientific Reports; 9(1) pg17240 Id PubMed 31754138 product.subtitle.productreviews Avis existants bovine embryos and derived embryonic stem cells This product works really good in in vitro bovine embryos and embryonic stem cells derived on MEFs. Perfect to validate pluripotency De: Nicolás | Date: 09/07/2020 × bovine embryos and derived embryonic stem cells This product works really good in in vitro bovine embryos and embryonic stem cells derived on MEFs. Perfect to validate pluripotency De: Nicolás | Date: 09/07/2020 Close