Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hsCD40L. Anti-Human sCD40L specific antibody was purified by affinity chromatography employing immobilized hsCD40L matrix.
E.coli derived Recombinant Human sCD40 Ligand (PeproTech catalog# 310-02)
To detect hsCD40 Ligand by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human sCD40 Ligand (500-P142GBt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsCD40 Ligand.
To detect hsCD40 Ligand by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsCD40 Ligand is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.