Antigen-affinitätsgereinigte Polyklonale Antikörper

Anti-Human sCD40 Ligand

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Product Details

Catalogue Number: 500-P142G
Description:
Anti-Human sCD40 Ligand
 

Source: Polyclonal Goat

Preparation: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hsCD40L. Anti-Human sCD40L specific antibody was purified by affinity chromatography employing immobilized hsCD40L matrix.

Immunogen: E.coli derived Recombinant Human sCD40 Ligand (PeproTech catalog# 310-02)

Sandwich ELISA: To detect hsCD40 Ligand by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human sCD40 Ligand (500-P142GBt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsCD40 Ligand.

Anti-Human sCD40 Ligand Sandwich ELISA

Western Blot: To detect hsCD40 Ligand by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsCD40 Ligand is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human sCD40 Ligand Western Blot Reduced Anti-Human sCD40 Ligand Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Aranburu, A
Title
TLR ligation triggers somatic hypermutation in transitional B cells inducing the generation of IgM memory B cells.
Citation
Journal of immunology (Baltimore, Md. : 1950); 185(12) pg7293-301
PubMed Id