Produced from sera of rabbits immunized with highly pure Recombinant Human BAFF. Anti-Human BAFF-specific antibody was purified by affinity chromatography and then biotinylated.
E.coli derived Recombinant Human BAFF (PeproTech catalog# 310-13)
To detect Human BAFF by sandwich ELISA (using 100μl/well) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human BAFF (500-P163) as a capture antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human BAFF.
To detect Human BAFF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents the detection limit for Recombinant Human BAFF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
1mg will be provided as 2x500μg.