Produced from sera of rabbits immunized with highly pure Recombinant Human BAFF. Anti-Human BAFF-specific antibody was purified by affinity chromatography employing an immobilized Human BAFF matrix.
E.coli derived Recombinant Human BAFF (PeproTech catalog# 310-13)
To detect Human BAFF by sandwich ELISA (using 100μl/well) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human BAFF (500-P163BT) as a detection antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human BAFF.
To detect Human BAFF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human BAFF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Additional applications tested on a lot‐to‐lot basis. Please contact Technical Support for more information.