Antigen Affinity Purified Polyclonal Antibodies

Anti-Human BAFF (Polyclonal Rabbit)

Product Details

Catalogue Number: 500-P163

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human BAFF. Anti-Human BAFF-specific antibody was purified by affinity chromatography employing an immobilized Human BAFF matrix.

Immunogen: E.coli derived Recombinant Human BAFF (PeproTech catalog# 310-13)

Sandwich ELISA: To detect Human BAFF by sandwich ELISA (using 100μl/well) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human BAFF (500-P163BT) as a detection antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human BAFF.

Anti-Human BAFF Sandwich ELISA

Western Blot: To detect Human BAFF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human BAFF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human BAFF Western Blot Unreduced Anti-Human BAFF Western Blot Reduced

Note:

Additional applications tested on a lot‐to‐lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Zhao, Y
Title
Tolerogenic Dendritic Cells Generated by BAFF Silencing Ameliorate Collagen-Induced Arthritis by Modulating the Th17/Regulatory T Cell Balance.
Citation
Journal of Immunology; 204(3) pg518-530
PubMed Id