Quality

PeproTech’s Quality Assurance ensures that all antibodies and ELISA Kits meet in-house product specifications and lot-to-lot comparability prior to release.

Polyclonal Antibodies

PeproTech’s polyclonal antibodies are purified through the isolation of specific polyclonal antibodies from antiserum by antigen affinity chromatography. This procedure exploits the specificity of the antibody-antigen interactions and typically yields >95% pure specific antibodies. Normally, the sera from host animals after immunization with cytokines, contain only small amounts (<5%) of cytokine-specific antibody which cannot be effectively isolated by standard purification procedures (e.g. ion exchange chromatography) or by non-antigen-specific affinity procedures, such as protein A/G affinity purification. 

The large quantities of unrelated IgGs found in these inferior preparations can considerably increase the background when the antibody is used in analytical procedures such as ELISA, neutralization, immunohistochemistry, and Western Blotting. Therefore, the use of the superior antigen affinity-purified antibody preparations can help alleviate background in these analytical procedures.

Biotinylated Antibodies

PeproTech’s biotinylated antibodies are produced from highly pure and specific antigen affinity-purified polyclonal antibodies, and are therefore ideal for use in any analytical procedures that require biotinylated antibodies.

Monoclonal Antibodies

PeproTech’s monoclonal antibodies are raised against full-length recombinant antigens and have been thoroughly screened for performance in a variety of applications.

ELISA Development Kits

PeproTech’s ELISA Development Kits contain the necessary key components for the quantitative measurement of natural and/or recombinant proteins in a sandwich ELISA format. Each kit contains a capture antibody, a biotinylated detection antibody, a calibrated antigen standard, the avidin-peroxidase conjugate, and a detailed protocol.

 

QC Testing Requirements for Antibodies

Antibody Content

Verified by UV Spectroscopy and SDS-PAGE.

ELISA

Tested by "Enzyme Linked Immunosorbent Assay" (ELISA) for antibody-antigen detection and quantification of the antigen, using a solid-phase substrate such as a polystyrene plate, enzyme-coupled reagents, and additional detection materials.

Endotoxin

Tested with the kinetic LAL (Limulus Amebocyte Lysate).

FACS (if applicable)

Tested by fluorescence activated cell sorting (FACS) flow cytometry.

Immunohistochemistry (IHC) (if applicable)

Tested with an immuno-coloring assay (immunoenzymatic or immunofluorescent) for specific antibody-antigen recognition in a tissue or cell sample, using an enzyme-coupled reagent and other detection materials. 

Neutralization (if applicable)

Tested to determine the antibody concentration required for a half-maximal inhibition [ND₅₀] of the biological activity of the corresponding antigen.

Sterility

All products are sterile filtered through a 0.2μm filter.

Western Blot

Tested by an immunoblot assay for antibody-antigen detection and quantification, using SDS-PAGE, nitrocellulose membrane transfer, an enzyme-coupled reagent, and other detection materials.

 

QC Testing Requirements for ELISA Development Kits

Cross Reactivity

Tested against closely related cytokine products to determine levels of antibody-antigen detection.

ELISA

Tested by “Enzyme Linked Immunosorbent Assay” (ELISA) for antibody-antigen detection and quantification of the antigen, using a solid-phase substrate such as a polystyrene plate, enzyme-coupled reagents, and additional detection materials.

Endotoxin

Capture, detection, and standard components tested with the kinetic LAL (Limulus Amebocyte Lysate).

Sterility

Capture, detection, and standard components are sterile filtered through a 0.2μm filter.