Full length and fully biologically active RUO cytokines are developed in-house by our experienced protein scientists. The process starts in our molecular biology labs with gene design and expression, to cell banking, and continues to fermentation/cell culture, purification and finally QC testing.
E.coli expressed proteins:
With over 15,000 sq. ft. of fermentation, protein processing and purification facilities, PeproTech is able to meet the demands of its clients.
Cell culture expressed proteins:
With state-of-the-art cell culture and protein purification facilities, PeproTech is also able to provide glycosylated, fully biologically active proteins.
PeproTech's Animal-Free product line is designed to minimize potential risks that can arise from trace amounts of animal-derived adventitious agents, such as viruses, TSE/BSE and unknowns.
PeproTech's E.coli-derived Animal-Free cytokines are manufactured in a dedicated Animal-Free facility under strict Animal-Free conditions where the production protocols have been modified to include only Animal-Free reagents and chemicals. All product contact material is also qualified as animal component-free.
Glycosylated and fully biologically active, PeproTech’s cell culture-derived Animal-Free Cytokines are manufactured in our NJ facility, using animal-free raw materials and expression systems consisting of serum-free, animal-free, chemically-defined media.
PeproTech's Animal-Free proteins maintain identical high biological activity and purity to the corresponding proteins that are produced using standard techniques that equal or enhance the performance of animal-derived media formulations. Products are lyophilized, with a minimum amount of salt, and without additives or preservatives added.
PeproTech’s RUO cytokines are subject to a rigorous set of quality control standards. Our Quality Assurance department ensures that the cytokines demonstrate consistent molecular weights, N-terminal sequences, purity and biological activity. This quality control is devoted to providing consistency between lots and the following qualities:
RUO cytokine products are verified by N-terminal sequence analysis, SDS-PAGE, Mass Spectrometry, and, where possible, by RP-HPLC, and Western Blot analyses versus standards.
Determined in the relevant in vitro and in vivo bioassay.
Tested by kinetic LAL (Limulus Amebocyte Lysate).
Verified by UV spectroscopy, SDS-PAGE and, where possible, by RP-HPLC.
Verified by SDS-PAGE and, where possible, by RP-HPLC.
All products are sterile filtered through a 0.2 micron filter.