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  4. Recombinant Human FGF-basic (154 a.a.)

Recombinant Human FGF-basic (154 a.a.)

Product Details

Catalogue Number: 100-18B
Description:
Recombinant Human FGF-basic (154 a.a.)

FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic is a non-glycosylated, heparin-binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland, liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4. Recombinant Human FGF-basic is a 17.2 kDa protein consisting of 154 amino acid residues.
Qualified for stem cell use.

Source: E.coli

Synonyms: Fibroblast Growth Factor-basic, FGF-2, HBGF-2, Prostatropin

AA Sequence: AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS

Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.

This product has been tested against the WHO standard. International Unit information can be found here.

Recombinant Human FGF-basic (154 a.a.) Biological Activity Graph

Calculated Molecular Weight: 17.2 kDa

Accession Number: P09038

Gene ID: 2247

Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug)

crossreactivity:
Alpaca, Bacteria, Chicken, Cow, Dog, Frog, Guinea Pig, Hamster, Horse, Human, Human + Hamster, Human + Mouse, Human + Rat, Human + Virus, Monkey, Mouse, Mouse + Chicken, Mouse + Pig, Mouse + Rat, Pig, Rabbit, Rat, Rat + Chicken, Sheep
Country Of Origin: USA

Not for human use.

Research Interest
Organoids Angiogenesis/Cardiovascular Bone, Skeletal, Cartilage Cancer Cell Culture FGF Superfamily Immune System Inflammation Neurobiology Stem Cells & Differentiation Wound Healing Diabetes/Weight Regulation

product.subtitle.recentcitations

First Author
Fu, H
Title
Thrombospondin 1 missense alleles induce extracellular matrix protein aggregation and TM dysfunction in congenital glaucoma.
Citation
The Journal of Clinical Investigation; 132(23)
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36453543
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Niu, Y
Title
Multiparametric and accurate functional analysis of genetic sequence variants using CRISPR-Select.
Citation
Nature Genetics; 54(12) pg1983-1993
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36471068
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Title
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Advanced science (Weinheim, Baden-Wurttemberg, Germany); 9(32) pge2203557
PubMed Id
36117048

product.subtitle.productreviews

Existing reviews
Excellent for iPS endothelial differentiation
Yet another excellent product by PeproTech! This recombinant human FGF is used at 20ng/mL with VEGF for iPS endothelial (EC) differentiation to generate high quality ECs.
From:  DIlip | Date:  13.07.2020
Very good for stem cell maintenance
Very good product for human stem cell and cancer stem cell maintenance and proliferation, of higher quality than similar product of competitors!
From:  stefania | Date:  05.08.2020
Another great iPSC differentiation growth factor!
I have found this product to remain exceptionally stable in media and give good, reproducible results when I am differentiating human iPSCs. Plus, the product ships quickly (which is important when I realize I am running low on growth factors). Thank you Peprotech for quality products and fast service!
From:  Kendall | Date:  26.08.2020
review for bFGF
We have been purchasing bFGF from Peprotech for the past 5 years and the product is very reliable. We've never had a problem. The tech support has been very helpful.
Thank you,
From:  Yingzi | Date:  03.08.2021
Great for organoids!
High quality product for patient tumor organoids.
From:  Julie-Ann | Date:  03.08.2021
Excellent product. We use it standardly for our primary cell cultures
Easy to use. We have used it in our standard TC medium for our primary cells for years.
From:  Helen | Date:  09.08.2021
Stem Cell Culture
Very consistent for culturing
From:  Danielle | Date:  24.08.2021
Constant quality for in vitro culture
Using in in vitro culture. Constant quality. Also swift delivery.
From:  KOHSUKE | Date:  24.08.2021
FGF, human basic recombinant, E. coli
Using the FGF in culture medium now for 6 years and very satisfied. Good quality and good customer support from Peprotech. Keep up the good work !
From:  Arjan | Date:  27.08.2021
Great recombinant protein
Works very nicely in combination with EGF to keep mouse ESC-derived NPCs alive and proliferating in culture (at 10 ng/ml concentration). Highly recommend.
From:  Epp | Date:  31.08.2021