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Antigen Affinity Purified Polyclonal Antibodies

Anti-Human G-CSF

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Product Details

Catalogue Number: 500-P43
Anti-Human G-CSF

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hG-CSF. Anti-Human G-CSF specific antibody was purified by affinity chromatography employing immobilized hG-CSF matrix.

Immunogen: E.coli derived Recombinant Human G-CSF (PeproTech catalog# 300-23)

Sandwich ELISA: To detect hG-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human G-CSF (500-P43Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hG-CSF.

Anti-Human G-CSF Sandwich ELISA

Western Blot: To detect hG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hG-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human G-CSF Western Blot Reduced Anti-Human G-CSF Western Blot Unreduced


Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Country Of Origin: USA

Not for human use.

Research Interest


First Author
Tian, L
Ubiquitin fusion expression and tissue-dependent targeting of hG-CSF in transgenic tobacco.
BMC Biotechnology; 11 pg91
PubMed Id
First Author
Kim, H K
G-CSF down-regulation of CXCR4 expression identified as a mechanism for mobilization of myeloid cells.
Blood; 108(3) pg812-20
PubMed Id