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Monoclonal Antibodies

Anti-Murine IL-12 (Monoclonal Rat)

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Product Details

Catalogue Number: 500-M59

Source: Monoclonal Rat

Preparation: Produced in Sprague Dawley rats immunized with highly pure Recombinant Murine IL-12. Anti-Murine IL-12-specific antibody was purified from cell culture by Protein G affinity chromatography.

Immunogen: CHO cell derived Recombinant Murine IL-12 p70 (PeproTech catalog# 210-12)

Sandwich ELISA: In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 1000pg/ml of Recombinant Murine IL-12 when used with PeproTech’s Biotinylated Antigen Affinity Purified anti-Murine IL-12 (500-P155GBT) as the detection antibody at a concentration of approximately 1.0-2.0 μg/ml.

Anti-Murine IL-12 Sandwich ELISA

Western Blot: To detect Murine IL-12 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine IL-12 is 1.0-2.0 ng/lane, under reducing conditions and 0.25-0.5 ng/lane, under non-reducing conditions.

Anti-Murine IL-12 Western Blot Reduced	Anti-Murine IL-12 Western Blot Unreduced


Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Country Of Origin: USA

Not for human use.

Research Interest


First Author
McElroy, D S
Use of the CD107 mobilization assay reveals that cytotoxic T lymphocytes with novel MHC-Ib restriction are activated during Listeria monocytogenes infection.
Journal of Immunological Methods; 328(1-2) pg45-52
PubMed Id
First Author
D'Orazio, S E
Cytosolic localization of Listeria monocytogenes triggers an early IFN-gamma response by CD8+ T cells that correlates with innate resistance to infection.
Journal of immunology (Baltimore, Md. : 1950); 177(10) pg7146-54
PubMed Id