Produced from sera of rabbits immunized with
highly pure recombinant Human TFF-2. Anti-Human
TFF-2 specific antibody was purified by affinity chromatography and then
E.coli-derived, 12.0 kDa Recombinant Human TFF-2 (PeproTech catalog# 300-59)
To detect Human TFF-2 by sandwich ELISA (using
100 μl/well) a concentration of 0.25 – 1.0 μg/ml of
this antibody is required. This biotinylated polyclonal antibody, in
conjunction with PeproTech’s Polyclonal Anti- Human TFF-2 (500-P312) as a
capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of
recombinant Human TFF-2.
To detect Human TFF-2 by Western Blot analysis
this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible
development reagents the detection limit for recombinant Human TFF-2 is 1.5 –
3.0 ng/lane, under either reducing or non-reducing conditions.
1mg will be provided as 2x500μg.