Produced from sera of rabbits immunized with highly pure recombinant Murine TRAIL. Anti-Murine TRAIL specific antibody was purified by affinity chromatography and then biotinylated.
E.coli-derived, 20.0 kDa Recombinant Murine TRAIL (PeproTech catalog# 315-19)
To detect Murine TRAIL by sandwich ELISA (using
100 ml/well) a concentration of 0.25 – 1.0 μg/ml of
this antibody is required. This biotinylated polyclonal antibody, in
conjunction with PeproTech’s Polyclonal Anti-Murine TRAIL (500-P303) as a
capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of
recombinant Murine TRAIL.
To detect Murine TRAIL by Western Blot analysis
this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. When used in conjunction with compatible
development reagents the detection limit for recombinant Murine TRAIL is 1.5 –
3.0 ng/lane, under either reducing or non-reducing conditions.
1mg will be provided as 2x500μg.