Neurotrophins

Recombinant Murine GDNF

Product Details

Catalogue Number: 450-44
Description:

GDNF is a disulfide-linked, homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin.  These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures.  GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors.  GDNF specifically promotes dopamine uptake and survival, and morphological differentiation of midbrain neurons.  Using a Parkinson’s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability.  The functional murine GDNF ligand is a disulfide-linked homodimer consisting of two 15.1 kDa polypeptide chains called monomers.  Each monomer contains seven conserved cysteine residues, including Cys-101, which is used for inter-chain disulfide bridging, and others that are involved in the intramolecular ring formation known as the cysteine-knot configuration. The calculated molecular weight of Recombinant Murine GDNF is 30.2 kDa.

Source: E.coli

Synonyms: Glial-Derived Neurotrophic Factor, ATF-1

AA Sequence (monomer): MSPDKQAAAL PRRERNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCESAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDNLV YHILRKHSAK RCGCI

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED50 was determined by the proliferation of rat C6 cells is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 106 units/mg.

Calculated Molecular Weight: 30.2 kDa

Accession Number: P48540

Gene ID: 14573

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Walter, J
Title
The Parkinson's-disease-associated mutation LRRK2-G2019S alters dopaminergic differentiation dynamics via NR2F1.
Citation
Cell Reports; 37(3) pg109864
PubMed Id
First Author
Rore, H
Title
Testicular somatic cell-like cells derived from embryonic stem cells induce differentiation of epiblasts into germ cells
Citation
Communications Biology; 4(1) pg802
PubMed Id
First Author
Hensel, N
Title
Impairment of the neurotrophic signaling hub B-Raf contributes to motoneuron degeneration in spinal muscular atrophy.
Citation
Proceedings of the National Academy of Sciences of the United States of America; 118(18)
PubMed Id