Anticorps Polyclonaux Purifiés par Affinité à l’Antigène

Anti-Human EGF Receptor (EGFR)

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Product Details

Catalogue Number: 500-P306

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits immunized with highly pure recombinant Human EGF Receptor. Anti-Human EGF Receptor specific antibody was purified by affinity chromatography employing an immobilized Human EGF Receptor matrix.

Immunogen: CHO cells derived Recombinant Human EGF Receptor (EGFR) (PeproTech catalog# 100-15R)

Sandwich ELISA: To detect Human EGF Receptor by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human EGF Receptor (500-P306Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human EGF Receptor.

Anti-Human EGF Receptor (EGFR) Sandwich ELISA

Western Blot: To detect Human EGF Receptor by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human EGF Receptor is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human EGF Receptor (EGFR) Western Blot Reduced Anti-Human EGF Receptor (EGFR) Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Piyush, T
Title
Interaction of galectin-3 with MUC1 on cell surface promotes EGFR dimerization and activation in human epithelial cancer cells.
Citation
Cell Death and Differentiation; 24(11) pg1937-1947
PubMed Id