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Anticorps Polyclonaux Purifiés par Affinité à l’Antigène

Anti-Human LIGHT

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Product Details

Catalogue Number: 500-P179
Anti-Human LIGHT

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hLIGHT . Anti-Human LIGHT specific antibody was purified by affinity chromatography employing immobilized hLIGHT matrix.

Immunogen: Insect cells derived Recombinant Human LIGHT (PeproTech catalog# 310-09B)

Sandwich ELISA: To detect hLIGHT by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human LIGHT (500-P179Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hLIGHT.

Anti-Human LIGHT Sandwich ELISA

Western Blot: To detect hLIGHT by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLIGHT is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human LIGHT Western Blot Reduced Anti-Human LIGHT Western Blot Unreduced


Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Country Of Origin: USA

Not for human use.

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