FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic exerts proliferative activity towards a wide range of cell types and consequently is used as a vital component in numerous cell culture media formulations, including stem cell media. FGF-basic loses its activity over time in solution at physiological temperatures and needs to be resupplemented periodically to maintain its performance. As a result of selected amino acid substitutions, Heat Stable FGF-basic retains full biological activity and also maintains full activity over extended periods of time in solution at 37°C. These properties make Heat Stable FGF-basic a superior choice for use in cell culture media applications. Heat Stable Recombinant Human FGF-basic is a 17.2 kDa protein consisting of 154 amino acids.
Heat Stable Recombinant Human FGF-basic (154 a.a.) was developed under a license from Enantis.
Fibroblast Growth Factor-basic, FGF-2, HBGF-2, Prostatropin
Thermostable variant of human FGF-basic (154 amino acids) with proprietary amino acid substitutions.
≥ 95% by SDS-PAGE gel and HPLC analyses.
Determined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED50 is 0.05-0.25 ng/ml.
Calculated Molecular Weight:
Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug)
Not for human use.