Produced in BALB/c mice immunized with highly pure Recombinant Human IFN-γ. Anti-Human IFN-γ-specific antibody was purified from cell culture by Protein A affinity chromatography.
E.coli derived Recombinant Human IFN-γ (PeproTech catalog# 300-02)
In a sandwich ELISA (assuming 100μl/well), a concentration of 4.0-8.0 μg/ml of this antibody will detect at least 500pg/ml of Recombinant Human IFN-γ when used with PeproTech’s Biotinylated Antigen Affinity Purified anti-Human IFN-γ (500-P32BT) as the detection antibody at a concentration of approximately 2.0- 4.0 μg/ml.
To detect Human IFN-γ by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IFN-γ is 1.0-2.0 ng/lane, under reducing or non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.