Animal-Free Recombinant Human GDNF 0 评论Submit a Review 产品详情 产品编号: AF-450-10 产品描述: GDNF is a disulfide-linked, homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors. GDNF specifically promotes dopamine uptake and survival, and morphological differentiation of midbrain neurons. Using a Parkinson’s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. The functional human GDNF ligand is a disulfide-linked homodimer consisting of two 15 kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, including Cys-101, which is used for inter-chain disulfide bridging, and others that are involved in the intramolecular ring formation known as the cysteine-knot configuration. The calculated molecular weight of Recombinant Human GDNF is 30.4 kDa. Manufactured using all Animal-Free reagents. Source: E.coli Synonyms: Glial-Derived Neurotrophic Factor, ATF-1 AA Sequence (monomer): MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by a cell proliferation assay using SH-SY5Y cells. The expected ED50 for this effect is 1.0-10.0 ng/ml. This product has been tested against the WHO standard. International Unit information can be found here. Calculated Molecular Weight: 30.4 kDa Accession Number: P39905 Gene ID: 2668 Endotoxin: Endotoxin level is < 0.01 ng/ug of protein (< 0.1 EU/ug) crossreactivity: Human, Monkey, Mouse References PubMed SDS CoA 搜索 Product Line Country Of Origin: USA Not for human use. Research Interest Organoids 血管生成/心血管 细胞培养 免疫系统 神经生物学 干细胞&分化 TGF-β超家族 product.subtitle.recentcitations 第一作者 Özcan, A 标题 Programmable RNA targeting with the single-protein CRISPR effector Cas7-11 文献引用 Nature; 597(7878) pg720-725 PubMed Id 34489594 第一作者 Brighi, C 标题 Novel fragile X syndrome 2D and 3D brain models based on human isogenic FMRP-KO iPSCs. 文献引用 Cell death & disease; 12(5) pg498 PubMed Id 33993189 第一作者 Ke, M 标题 Azoramide protects iPSC-derived dopaminergic neurons with PLA2G6 D331Y mutation through restoring ER function and CREB signaling 文献引用 Cell Death & Disease; 11(2) pg130 PubMed Id 32071291