Antigen Affinity Purified Polyclonal Antibodies

Anti-Human sTNF Receptor Type I

Product Details

Catalog Number: 500-P143

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hsTNF-receptor. Anti-Human sTNF-receptor specific antibody was purified by affinity chromatography employing immobilized hsTNF-receptor matrix.

Immunogen: E.coli derived Recombinant Human sTNF Receptor Type I (PeproTech catalog# 310-07)

Sandwich ELISA: To detect hsTNF-receptor by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human sTNF-receptor (500-P143Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsTNF-receptor.

Anti-Human sTNF Receptor Type I Sandwich ELISA

Western Blot: To detect hsTNF-receptor by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTNF-receptor is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human sTNF Receptor Type I Western Blot Reduced Anti-Human sTNF Receptor Type I Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Research Interest

Country Of Origin: USA

Research Use Only: Not for use in diagnostic or therapeutic procedures.