Recombinant Human TPO 1 ReviewsSubmit a Review Product Details Catalogue Number: 300-18 Description: TPO is a lineage-specific growth factor produced in the liver, kidney and skeletal muscle. It stimulates the proliferation and maturation of megakaryocytes, and promotes increased circulating levels of platelets in vivo. TPO signals through the c-mpl receptor, and acts as an important regulator of circulating platelets. Human and murine TPO exhibit cross-species reactivity. The human TPO gene encodes for a 353 amino acid glycoprotein, which contains a 21 amino acid signal sequence, a 15 amino acid erythropoietin-like domain, and a highly glycosylated 179 amino acid C-terminal domain. Recombinant Human TPO is a fully biologically active 174 amino acid polypeptide (18.6 kDa), which contains the erythropoietin-like domain of the full length TPO protein. Source: E.coli Synonyms: Thrombopoietin, Megakaryocyte colony-stimulating factor, c-MPL Ligand, MGDF AA Sequence: SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of human MO7e cells was found to be ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg. Calculated Molecular Weight: 18.6 kDa Accession Number: P40225 Gene ID: 7066 crossreactivity: Human, Human + Hamster, Human + Monkey, Human + Mouse, Monkey, Mouse, Mouse + Virus, Rat References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Cell Culture Immune System Stem Cells & Differentiation Wound Healing product.subtitle.recentcitations First Author Zhou, Y Title Megakaryocytes participate in the occurrence of bleomycin-induced pulmonary fibrosis. Citation Cell Death & Disease; 10(9) pg648 PubMed Id 31501415 First Author Radtke, S Title Human multipotent hematopoietic progenitor cell expansion is neither supported in endothelial and endothelial/mesenchymal co-cultures nor in NSG mice. Citation Scientific Reports; 9(1) pg12914 PubMed Id 31501490 First Author Kauts, M Title Rapid Mast Cell Generation from Gata2 Reporter Pluripotent Stem Cells. Citation Stem Cell Reports; 11(4) pg1009-1020 PubMed Id 30197119 product.subtitle.productreviews Existing reviews TPO dans la culture Cellules souches pluripotentes J'ai utilise de la TPOhu pour la différenciation des cellules souches pluripotentes humaines vers l'hemangioblaste et les cellules hématopoïètiques.Je suis très satisfaite de ce produit (et depuis plusieures années) From: Maite | Date: 29.07.2020 × TPO dans la culture Cellules souches pluripotentes J'ai utilise de la TPOhu pour la différenciation des cellules souches pluripotentes humaines vers l'hemangioblaste et les cellules hématopoïètiques.Je suis très satisfaite de ce produit (et depuis plusieures années) From: Maite | Date: 29.07.2020 Close
