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Recombinant Human TPO

Product Details

Catalogue Number: 300-18
Description:
Recombinant Human TPO

TPO is a lineage-specific growth factor produced in the liver, kidney and skeletal muscle. It stimulates the proliferation and maturation of megakaryocytes, and promotes increased circulating levels of platelets in vivo. TPO signals through the c-mpl receptor, and acts as an important regulator of circulating platelets. Human and murine TPO exhibit cross-species reactivity. The human TPO gene encodes for a 353 amino acid glycoprotein, which contains a 21 amino acid signal sequence, a 15 amino acid erythropoietin-like domain, and a highly glycosylated 179 amino acid C-terminal domain. Recombinant Human TPO is a fully biologically active 174 amino acid polypeptide (18.6 kDa), which contains the erythropoietin-like domain of the full length TPO protein.

Source: E.coli

Synonyms: Thrombopoietin, Megakaryocyte colony-stimulating factor, c-MPL Ligand, MGDF

AA Sequence: SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of human MO7e cells was found to be ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.

Calculated Molecular Weight: 18.6 kDa

Accession Number: P40225

Gene ID: 7066

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Thomas, G E
Title
The metabolic enzyme hexokinase 2 localizes to the nucleus in AML and normal haematopoietic stem and progenitor cells to maintain stemness.
Citation
Nature Cell Biology; 24(6) pg872-884
PubMed Id
First Author
Tvorogov, D
Title
Targeting human CALR-mutated MPN progenitors with a neoepitope-directed monoclonal antibody.
Citation
EMBO reports; 23(4) pge52904
PubMed Id
First Author
Wang, Y
Title
Using Cryo-ET to distinguish platelets during pre-acute myeloid leukemia from steady state hematopoiesis.
Citation
Communications Biology; 5(1) pg72
PubMed Id

product.subtitle.productreviews

Existing reviews
TPO dans la culture Cellules souches pluripotentes
J'ai utilise de la TPOhu pour la différenciation des cellules souches pluripotentes humaines vers l'hemangioblaste et les cellules hématopoïètiques.
Je suis très satisfaite de ce produit (et depuis plusieures années)
From:  Maite | Date:  29.07.2020