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Recombinant Murine M-CSF

Product Details

Catalogue Number: 315-02
Description:
Recombinant Murine M-CSF

M-CSF is a potent hematopoietic factor produced by a variety of cells, including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. It is a key regulator of cellular proliferation, differentiation, and survival for blood monocytes, tissue macrophages, and their respective progenitor cells. M-CSF has been shown to play important roles in modulating dermal thickness and fertility. M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates hematopoietic recovery after bone marrow transplantation. Human M-CSF is reactive in murine systems, but the murine molecule exhibits no activity on human cells. Recombinant Murine M-CSF is a 36.4 kDa homodimeric protein consisting of two 156 amino acid polypeptide subunits.

Source: E.coli

Synonyms: Macrophage Colony Stimulating Factor, CSF-1, MGI-IM

AA Sequence (monomer): MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of M-NFS-60 cells is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.

Calculated Molecular Weight: 36.4 kDa

Accession Number: P07141

Gene ID: 12977

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Sharma, A
Title
Glycolipid Metabolite β-Glucosylceramide Is a Neutrophil Extracellular Trap-Inducing Ligand of Mincle Released during Bacterial Infection and Inflammation.
Citation
Journal of immunology (Baltimore, Md. : 1950); 209(2) pg391-400
PubMed Id
First Author
Huang, M
Title
miR-134-5p inhibits osteoclastogenesis through a novel miR-134-5p/Itgb1/MAPK pathway.
Citation
The Journal of Biological Chemistry; 298(7) pg102116
PubMed Id
First Author
Li, X
Title
Sorafenib inhibits LPS-induced inflammation by regulating Lyn-MAPK-NF-kB/AP-1 pathway and TLR4 expression.
Citation
Cell Death Discovery; 8(1) pg281
PubMed Id

product.subtitle.productreviews

Existing reviews
Differencitaion of bone marrow progenitors in bone marrow derived macrophages
I used murine M-CSF to differentiate bone marrow progenitors in bone marrow derived macrophages.
From:  Laura | Date:  28.07.2020
MCSF
Good product. Works well to generate macrophages from BM.
From:  Kevin | Date:  26.08.2020
Excellent product
I have used this product for differentiating bone marrow-derived monocytes to macrophages. It works very well based on visual observations as well as flow cytometry data on macrophage surface protein expression. I use it very often for my project.
From:  Kiran | Date:  26.08.2020
M-CSF for maintaining mouse microglial lines
I have been using murine M-CSF for maintaining mouse microglial lines, EOC20. And it worked perfectly. Definitely  recommend.
From:  Yongjin | Date:  26.08.2020