Anti-Human IL-7 0 ReviewsSubmit a Review Product Details Catalog Number: 500-P27 Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure recombinant Human IL-7. Anti-Human IL-7 specific antibody was purified by affinity chromatography employing immobilized Human IL-7 matrix. Immunogen: E.coli derived Recombinant Human IL-7 (PeproTech catalog# 200-07) Sandwich ELISA: To detect Human IL-7 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human IL-7 (500-P27Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-7. Western Blot: To detect Human IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-7 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Note: Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information. Cross Reactivity Cited in References: Human References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Cancer Inflammation Stem Cells & Differentiation Recent Citations First Author Giuliani, N Title Myeloma cells block RUNX2/CBFA1 activity in human bone marrow osteoblast progenitors and inhibit osteoblast formation and differentiation. Citation Blood; 106(7) pg2472-83 PubMed ID 15933061 First Author Giuliani, N Title Human myeloma cells stimulate the receptor activator of nuclear factor-kappa B ligand (RANKL) in T lymphocytes: a potential role in multiple myeloma bone disease. Citation Blood; 100(13) pg4615-21 PubMed ID 12393684