Produced in BALB/c mice immunized with highly pure Recombinant Rat IL-10. Anti-Rat IL-10-specific antibody was purified from tissue culture by Protein A affinity chromatography.
E.coli derived Recombinant Rat IL-10 (PeproTech catalog# 400-19)
In a sandwich ELISA (assuming 100μl/well), a concentration of 8.0-16.0 μg/ml of this antibody will detect at least 500pg/ml of Recombinant Rat IL-10 when used with PeproTech’s Biotinylated Antigen Affinity Purified anti-Rat IL-10 (500-P139BT) as the detection antibody at a concentration of approximately 0.5-1.0 μg/ml.
To detect Rat IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat IL-10 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.