Growth Factors & Cytokines

Recombinant Human sIL-6 Receptor α (CHO derived)

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Product Details

Catalogue Number: 200-06RC
Description:

IL-6 mediates its biological effects through the type I IL-6 receptor system that consists of two chains, IL-6Rα and gp130.  While the IL-6Rα chain is the binding component specific to IL-6, the gp130 chain only transmits signals of IL-6 when bound to IL-6Rα.  The gp130 can also transmit signals from LIF, OSM, CNTF, IL-11 and CT-1 in conjunction with other receptor subunits.  The low-affinity binding site for IL-6 is composed of IL-6Rα alone.  IL-6Rα is expressed in a wide range of cells, including T cells, fibroblasts and macrophages.  Soluble IL-6Rα, which consists of only the extracellular domain of the IL-6Rα chain, acts as an agonist of IL-6 activity at low concentrations. PeproTech's CHO cell-derived Recombinant Human sIL-6 Receptor α is a 37.9 kDa glycoprotein corresponding to 339 amino acid residues of the extracellular domain of IL-6Rα. As a result of glycosylation, Recombinant Human sIL-6 Receptor α migrates with an apparent molecular mass of approximately 57-70 kDa by SDS-PAGE gel, under reducing conditions.

Source: CHO cells

Synonyms: soluble IL-6 receptor alpha, B cell stimulatory factor-2, CD126

AA Sequence: LAPRRCPAQE VARGVLTSLP GDSVTLTCPG VEPEDNATVH WVLRKPAAGS HPSRWAGMGR RLLLRSVQLH DSGNYSCYRA GRPAGTVHLL VDVPPEEPQL SCFRKSPLSN VVCEWGPRST PSLTTKAVLL VRKFQNSPAE DFQEPCQYSQ ESQKFSCQLA VPEGDSSFYI VSMCVASSVG SKFSKTQTFQ GCGILQPDPP ANITVTAVAR NPRWLSVTWQ DPHSWNSSFY RLRFELRYRA ERSKTFTTWM VKDLQHHCVI HDAWSGLRHV VQLRAQEEFG QGEWSEWSPE AMGTPWTESR SPPAENEVST PMQALTTNKD DDNILFRDSA NATSLPVQD

Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by its ability to intensify the IL-6 induced growth inhibition of mouse M1 cells.  The expected ED50 is ≤ 5.0 ng/ml, in the presence of 20 ng/ml of rhIL-6.

Calculated Molecular Weight: 37.9 kDa

Accession Number: P08887

Gene ID: 3570

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Zhang, B
Title
CD127 imprints functional heterogeneity to diversify monocyte responses in inflammatory diseases.
Citation
The Journal of Experimental Medicine; 219(2)
PubMed Id
First Author
Bent, E H
Title
Microenvironmental IL-6 inhibits anti-cancer immune responses generated by cytotoxic chemotherapy.
Citation
Nature Communications; 12(1) pg6218
PubMed Id
First Author
Uyama, N
Title
Anti-interleukin-6 receptor antibody treatment ameliorates postoperative adhesion formation.
Citation
Scientific Reports; 9(1) pg17558
PubMed Id